Application
NS5 (JEV) antibody detects NS5 (JEV) protein by Western blot analysis. Un-infected (-) and infected (+, JEV infection) BHK-21 whole cell extracts (5 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NS5 (JEV) antibody at a dilution of 1:3000.Application
NS5 protein (Japanese encephalitis virus) antibody detects NS5 protein (Japanese encephalitis virus) by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Japanese encephalitis virus (right) were fixed in methanol. Green: NS5 (Japanese encephalitis virus) protein stained by NS5 protein (Japanese encephalitis virus) antibody diluted at 1:3000. Blue: Hoechst 33342 staining.| Product Name | NS5 (JEV) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to non-structural protein NS5 (Japanese encephalitis virus)The small proteins NS2A, NS4A and NS4B are hydrophobic, suggesting a possible membrane-related function. Non-structural protein 2B is a required cofactor for the serine protease function of NS3. Serine protease NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS3 serine protease, in association with NS2B, cleaves the polyprotein. RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome, and performs the capping of genomes in the cytoplasm. NS5 methylates viral RNA cap at guanine N-7 and ribose 2'-O positions. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Japanese Encephalitis Virus |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | non-structural protein NS5 (Japanese encephalitis virus) |
|---|---|
| Molecular Weight(MW) | 103 kDa (note) |
Application
NS5 (JEV) antibody detects NS5 (JEV) protein by Western blot analysis. Un-infected (-) and infected (+, JEV infection) BHK-21 whole cell extracts (5 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NS5 (JEV) antibody at a dilution of 1:3000.
Application
NS5 protein (Japanese encephalitis virus) antibody detects NS5 protein (Japanese encephalitis virus) by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Japanese encephalitis virus (right) were fixed in methanol. Green: NS5 (Japanese encephalitis virus) protein stained by NS5 protein (Japanese encephalitis virus) antibody diluted at 1:3000. Blue: Hoechst 33342 staining.| Positive Control | JEV infected BHK-21 cells |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
