Application
NSD3 antibody [C1C2], Internal detects NSD3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NSD3 protein stained by NSD3 antibody [C1C2], Internal diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.Application
Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: Hela 5% SDS PAGE diluted at 1:1000| Product Name | NSD3 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NSD3 (Wolf-Hirschhorn syndrome candidate 1-like 1)This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. The function of the protein has not been determined. Two alternatively spliced variants have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NSD3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | BovineBovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp667H044 antibody FLJ20353 antibody MGC126766 antibody MGC142029 antibody NSD3 antibody pp14328 antibody WHSC1L1 antibody histone-lysine N-methyltransferase NSD3 antibody WHSC1-like 1 isoform 9 with methyltransferase activity to lysine antibody |
|---|---|
| Molecular Weight(MW) | 162 kDa (note) |
| Cellular Localization | Nucleus |
Application
NSD3 antibody [C1C2], Internal detects NSD3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NSD3 protein stained by NSD3 antibody [C1C2], Internal diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: Hela 5% SDS PAGE diluted at 1:1000| Positive Control | 293T , HeLa , Raji , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
