Application
Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NSF antibody diluted at 1:1000.Application
NSF antibody detects NSF protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. NSF antibody diluted at 1:500.Application
Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE diluted at 1:1000Application
Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NSF antibody diluted at 1:1000.| Product Name | NSF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NSF (N-ethylmaleimide-sensitive factor) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NSF. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Pig, Chicken, BovineXenopus laevis (88%), Pig (97%), Chicken (91%), Bovine (99%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | SKD2 antibody NSF antibody vesicle-fusing ATPase antibody N-ethylmaleimide-sensitive factor-like protein antibody vesicular-fusion protein NSF antibody NEM-sensitive fusion protein antibody N-ethylmaleimide-sensitive fusion protein antibody N-ethylm |
|---|---|
| Molecular Weight(MW) | 83 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NSF antibody diluted at 1:1000.
Application
NSF antibody detects NSF protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. NSF antibody diluted at 1:500.
Application
Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE diluted at 1:1000
Application
Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NSF antibody diluted at 1:1000.| Positive Control | 293T , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
