Application
NSFL1C antibody [N1C2] detects NSFL1C protein at cytoplasm and nucleus on mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. NSFL1C antibody [N1C2] diluted at 1:500.Application
Immunoprecipitation of NSFL1C protein from A431 whole cell extracts using 5 μg of NSFL1C antibody [N1C2] . Western blot analysis was performed using NSFL1C antibody [N1C2] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Sample (50 µg of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded differential N/T pair Lung SCC cancer xenograft, using NSFL1C antibody at 1:100 dilution.Application
Sample(30 µg whole cell lysate) A:293T B:A431 C:H1299 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | NSFL1C antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NSFL1C (NSFL1 (p97) cofactor (p47))N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NSFL1C. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Pig, Chicken, Rhesus Monkey, BovineRat (96%), Xenopus laevis (83%), Pig (98%), Chicken (87%), Rhesus Monkey (100%), Bovine (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC3347 antibody UBX1 antibody UBXD10 antibody UBXN2C antibody dJ776F14.1 antibody p47 antibody NSFL1C antibody NSFL1 cofactor p47 antibody SHP1 homolog antibody UBX domain-containing protein 2C antibody p97 cofactor p47 antibody NSFL1 (p97) cof |
|---|---|
| Molecular Weight(MW) | 41 kDa (note) (2) |
| Cellular Localization | Nucleus (By similarity) , Golgi apparatus , Golgi stack (By similarity) |
Application
NSFL1C antibody [N1C2] detects NSFL1C protein at cytoplasm and nucleus on mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. NSFL1C antibody [N1C2] diluted at 1:500.
Application
Immunoprecipitation of NSFL1C protein from A431 whole cell extracts using 5 μg of NSFL1C antibody [N1C2] . Western blot analysis was performed using NSFL1C antibody [N1C2] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Sample (50 µg of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded differential N/T pair Lung SCC cancer xenograft, using NSFL1C antibody at 1:100 dilution.
Application
Sample(30 µg whole cell lysate) A:293T B:A431 C:H1299 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
