Rabbit polyclonal antibody to NSMCE1 (non-SMC element 1 homolog (S. cerevisiae))Probable component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks.
Immunogen
Recombinant protein encompassing a sequence within the center region of human NSMCE1. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
IHC-P,WB
*Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity
Human,Monkey,Mouse,Rat
Predicted Reactivity
Mouse, Rat, BovineMouse (90%), Rat (92%), Bovine (90%)
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
NSE1 antibody, NSMCE1 antibody, non-structural maintenance of chromosomes element 1 homolog antibody, non-SMC element 1 homolog (S. cerevisiae) antibody
Target
NSMCE1
Molecular Weight(MW)
31 kDa (note)
Cellular Localization
Nucleus
Application
Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using NSMCE1 antibody at 1:500 dilution.
Application
Sample(30 ug whole cell lysate) A:Raji 12% SDS PAGE diluted at 1:2000
Positive Control
293T , Raji
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.