Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using NTAN1 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE diluted at 1:1000| Product Name | NTAN1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NTAN1 (N-terminal asparagine amidase)Side-chain deamidation of N-terminal asparagine residues to aspartate. Required for the ubiquitin-dependent turnover of intracellular proteins that initiate with Met-Asn. These proteins are acetylated on the retained initiator methionine and can subsequently be modified by the removal of N-acetyl methionine by acylaminoacid hydrolase (AAH). Conversion of the resulting N-terminal asparagine to aspartate by PNAD renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. This enzyme does not act on substrates with internal or C-terminal asparagines and does not act on glutamine residues in any position. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NTAN1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, BovineMouse (94%), Rat (95%), Pig (93%), Bovine (93%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp666E058 antibody NTAN1 antibody PNAA antibody protein NTN-amidase antibody protein NH2-terminal asparagine deamidase antibody protein NH2-terminal asparagine amidohydrolase antibody protein N-terminal Asn amidase antibody PNAD antibody protein |
|---|---|
| Molecular Weight(MW) | 35 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using NTAN1 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE diluted at 1:1000| Positive Control | 293T , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
