Application
NTE antibody detects NTE protein at cytosol on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. NTE antibody dilution: 1:200.Application
NTE antibody detects NTE protein at cytosol on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. NTE antibody dilution: 1:200.Application
NTE antibody detects NTE protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NTE antibody diluted by 1:1000.Application
NTE antibody detects NTE protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 5 % SDS-PAGE, and the membrane was blotted with NTE antibody diluted by 1:1000.| Product Name | NTE antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NTE (patatin-like phospholipase domain containing 6)This gene encodes a phospholipase that deacetylates intracellular phosphatidylcholine to produce glycerophosphocholine. It is thought to function in neurite outgrowth and process elongation during neuronal differentiation. The protein is anchored to the cytoplasmic face of the endoplasmic reticulum in both neurons and non-neuronal cells. Mutations in this gene result in autosomal recessive spastic paraplegia, and the protein is the target for neurodegeneration induced by organophosphorus compounds and chemical warfare agents. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human NTE. The exact sequence is proprietary. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB:1:500~1:2000 IHC-P:1:50~1:200 |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Chicken, BovineChicken (94%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | patatin-like phospholipase domain containing 6 NTEMND sws SPG39 PNPLA6 iPLA2delta |
|---|---|
| Target | NTE |
| Molecular Weight(MW) | 150 kDa (note) (6) |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass type I membrane protein; Cytoplasmic side |
Application
NTE antibody detects NTE protein at cytosol on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. NTE antibody dilution: 1:200.
Application
NTE antibody detects NTE protein at cytosol on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. NTE antibody dilution: 1:200.
Application
NTE antibody detects NTE protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NTE antibody diluted by 1:1000.
Application
NTE antibody detects NTE protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 5 % SDS-PAGE, and the membrane was blotted with NTE antibody diluted by 1:1000.| Positive Control | U87-MG , SK-N-SH , IMR-32 , SK-N-AS , rat brain |
|---|---|
| Application Notes | WB:1:500~1:2000 IHC-P:1:50~1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
