Rabbit Polyclonal antibody to Ntla (no tail a)Involved in the transcriptional regulation of genes required for mesoderm differentiation, including itself. Indispensable for the formation of the notochord and the tail structure. Functions together with tbx16/spadetail in development of trunk and tail mesoderm. Functions by itself early in development to repress medial floor plate and promote notochord fate but at later times, functions together with tbx16/spadetail to promote medial floor plate formation. Acts in a parallel pathway to, but cooperates with, non-canonical wnt-signaling during tail formation. Required for the morphogenesis of Kupffer's vesicle and regulates left-right asymmetry.
Immunogen
Recombinant protein encompassing a sequence within the center region of zebrafish Ntla. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
IHC-W
*Optimal dilutions/concentrations should be determined by the researcher.
:
Species Reactivity
Zebra Fish
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Target Information
Gene Synonyms
protein T homolog A antibody t protein homolog A antibody T-box protein ZfT antibody brachyury protein homolog A antibody no tail protein A antibody T antibody ZF-NTL antibody ZF-T antibody cb240 antibody id:ibd5074 antibody n
Target
no tail a
Molecular Weight(MW)
46 KDa (note)
Cellular Localization
Nucleus
Application
Application
ntla antibody detects ntla protein on whole-mount zebrafish embryos by immunohistochemical analysis. Sample: Paraformaldehyde-fixed zebrafish embryos. ntla antibody dilution: 1:200.
Positive Control
Zebrafish embryo
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.
:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.