Application
NUCB2 antibody detects NUCB2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NUCB2 antibody diluted at 1:1000.Application
NUCB2 antibody detects NUCB2 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NUCB2 protein stained by NUCB2 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Immunoprecipitation of NUCB2 protein from 293T whole cell extracts using 5 μg of NUCB2 antibody . Western blot analysis was performed using NUCB2 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
NUCB2 antibody detects NUCB2 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. NUCB2 antibody diluted at 1:500.| Product Name | NUCB2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NUCB2Nucleobindin-2 is a calcium-binding EF-hand protein.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human NUCB2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, BovineMouse (84%), Bovine (84%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NEFA nucleobindin 2 |
|---|---|
| Target | NUCB2 |
| Molecular Weight(MW) | 50 kDa (note) |
| Cellular Localization | Membrane; Peripheral membrane protein , Cytoplasm , Secreted |
Application
NUCB2 antibody detects NUCB2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NUCB2 antibody diluted at 1:1000.
Application
NUCB2 antibody detects NUCB2 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NUCB2 protein stained by NUCB2 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Immunoprecipitation of NUCB2 protein from 293T whole cell extracts using 5 μg of NUCB2 antibody . Western blot analysis was performed using NUCB2 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
NUCB2 antibody detects NUCB2 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. NUCB2 antibody diluted at 1:500.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
