Application
Immunofluorescence analysis of methanol-fixed HeLa, using Nucleobindin 1 antibody at 1:200 dilution.Application
Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE diluted at 1:1000Application
Nucleobindin 1 antibody [N3C3] detects Nucleobindin 1 protein at Golgi apparatus by immunofluorescent analysis. Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nucleobindin 1 protein stained by Nucleobindin 1 antibody [N3C3] diluted at 1:500. Red: FIS1, a mitochondria marker, stained by FIS1 antibody [GT9810] diluted at 1:20000. Blue: Hoechst 33342 staining.Application
Nucleobindin 1 antibody [N3C3] detects Nucleobindin 1 protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Nucleobindin 1 antibody [N3C3] dilution: 1:500.Application
Nucleobindin 1 antibody [N3C3] detects Nucleobindin 1 protein at cytosol on mouse brain stem by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain stem. Nucleobindin 1 antibody [N3C3] dilution: 1:500.| Product Name | Nucleobindin 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Nucleobindin 1 (nucleobindin 1)This gene encodes a member of a small calcium-binding EF-hand protein family. The encoded protein is thought to have a key role in Golgi calcium homeostasis and Ca(2+)-regulated signal transduction events. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Nucleobindin 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, BovineRat (85%), Pig (91%), Bovine (91%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CALNUC antibody DKFZp686A15286 antibody FLJ40471 antibody NUC antibody NUCB1 antibody nucleobindin-1 antibody nucleobindin 1 antibody |
|---|---|
| Molecular Weight(MW) | 54 kDa (note) (2) |
| Cellular Localization | Golgi apparatus , cis-Golgi network membrane; Peripheral membrane protein; Lumenal side (By similarity) , Cytoplasm (By similarity) |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using Nucleobindin 1 antibody at 1:200 dilution.
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE diluted at 1:1000
Application
Nucleobindin 1 antibody [N3C3] detects Nucleobindin 1 protein at Golgi apparatus by immunofluorescent analysis. Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nucleobindin 1 protein stained by Nucleobindin 1 antibody [N3C3] diluted at 1:500. Red: FIS1, a mitochondria marker, stained by FIS1 antibody [GT9810] diluted at 1:20000. Blue: Hoechst 33342 staining.
Application
Nucleobindin 1 antibody [N3C3] detects Nucleobindin 1 protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Nucleobindin 1 antibody [N3C3] dilution: 1:500.
Application
Nucleobindin 1 antibody [N3C3] detects Nucleobindin 1 protein at cytosol on mouse brain stem by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain stem. Nucleobindin 1 antibody [N3C3] dilution: 1:500.| Positive Control | A549 , HeLa , HepG2 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
