Application
ICC/IF analysis of NT-2 cells using NCL antibody at 1:500. Red: NCL Blue:DAPIApplication
WB analysis of NT-2 cells using NCL antibody at 2μg/ml.Application
IHC-P analysis of human kidney tissue using NCL antibodyApplication
WB analysis of Daudi cells using NCL antibody at 1.25μg/ml. Lane A: marker Lane B: Daudi cells| Product Name | Nucleolin antibody, C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NucleolinNucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthetic peptide corresponding to a C-terminal region of Human NCL |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF,IHC-P,WB |
| WB:1:500~1:1000 ICC/IF:1:100~1:500 IHC-p:1:50~1:100 *Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Predicted Reactivity | Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 93%; Rabbit: 100%; Rat: 100% |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | NCL , C23 |
|---|---|
| Target | NCL |
| Molecular Weight(MW) | 77 KDa (note) |
Application
ICC/IF analysis of NT-2 cells using NCL antibody at 1:500. Red: NCL Blue:DAPI
Application
WB analysis of NT-2 cells using NCL antibody at 2μg/ml.
Application
IHC-P analysis of human kidney tissue using NCL antibody
Application
WB analysis of Daudi cells using NCL antibody at 1.25μg/ml. Lane A: marker Lane B: Daudi cells| Positive Control | Daudi , NT-2 |
|---|---|
| Application Notes | WB:1:500~1:1000 ICC/IF:1:100~1:500 IHC-p:1:50~1:100 *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
