Application
Confocal immunofluorescence analysis of Hela (left) and NTERA-2 (right) cells using NPM mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.Application
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissues, showing nuclear localization using anti-NPM mAb with DAB staining.Application
Western blot analysis using anti-NPM mAb against SMMC-7721 (1), HepG2 (2), Hela (3) and HEK293 (4) cell lysate.| Product Name | Nucleophosmin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [7H10B9] to NucleophosminThis gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009] |
| Immunogen | The immunogen corresponding to human NPM expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 7H10B9 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | HumanMonkey |
| Concentration | 1mg/ml |
| Gene Synonyms | B23 NPM NPM1 |
|---|---|
| Target | Nucleophosmin |
| Molecular Weight(MW) | 33 kDa (note) |
| Cellular Localization | Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Has been found in the cytoplasm in patients with primary acute myelogenous leukemia (AML), but not with secondary AML. Can shuttle |
Application
Confocal immunofluorescence analysis of Hela (left) and NTERA-2 (right) cells using NPM mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
Application
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissues, showing nuclear localization using anti-NPM mAb with DAB staining.
Application
Western blot analysis using anti-NPM mAb against SMMC-7721 (1), HepG2 (2), Hela (3) and HEK293 (4) cell lysate.| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
