Application
Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of methanol-fixed HeLa, using Nucleoside phosphorylase antibody at 1:500 dilution.| Product Name | Nucleoside phosphorylase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Nucleoside phosphorylase (purine nucleoside phosphorylase)This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Nucleoside phosphorylase. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rhesus Monkey, BovineMouse (85%), Rhesus Monkey (95%), Bovine (88%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ94043 antibody FLJ97288 antibody FLJ97312 antibody MGC117396 antibody MGC125915 antibody MGC125916 antibody NP antibody PRO1837 antibody PUNP antibody PNP antibody purine nucleoside phosphorylase antibody inosine phosphorylase antibody purin |
|---|---|
| Molecular Weight(MW) | 32 kDa (note) (2) |
Application
Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of methanol-fixed HeLa, using Nucleoside phosphorylase antibody at 1:500 dilution.| Positive Control | Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
