Application
Immunoprecipitation of NUF2 protein from HeLa whole cell extracts using 5 μg of NUF2 antibody [GT644] . Western blot analysis was performed using NUF2 antibody [GT644] . EasyBlot anti-Mouse IgG was used as a secondary reagent.Application
HeLa whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NUF2 antibody [GT644] diluted at 1:1000.| Product Name | NUF2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT644] to NUF2 (NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae))This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq] |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | GT644 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | NUF2 NUF2R NUF2 NDC80 kinetochore complex component homolog (S. cerevisiae) CT106 |
|---|---|
| Target | CDCA1 |
| Molecular Weight(MW) | 54 kDa (note) |
| Cellular Localization | Nucleus , Kinetochore |
Application
Immunoprecipitation of NUF2 protein from HeLa whole cell extracts using 5 μg of NUF2 antibody [GT644] . Western blot analysis was performed using NUF2 antibody [GT644] . EasyBlot anti-Mouse IgG was used as a secondary reagent.
Application
HeLa whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NUF2 antibody [GT644] diluted at 1:1000.| Positive Control | HeLa , HeLa NE |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
