Application
Immunocytochemistry/Immunofluorescence: NuMA Antibody [ - Immunofluorescent image of human HeLa cells fixed with 3.5% formaldehyde and stained with at 1:1000 dilution. The staining pattern shows the typical pattern for NuMA in the cell nucleus during interphase and on spindles during mitosisApplication
Western Blot: NuMA Antibody [ - Western blot analysis of NuMA expression in Cos7 whole cell lysate| Product Name | NuMA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to NuMA (Nuclear Mitotic Apparatus)NuMA (nuclear mitotic apparatus) is a long coil-coiled protein that plays a role in the interphase and mitosis phases of a cell cycle. In interphase, it has been hypothesized to play a structural role in the nucleoskeleton that may be important in nuclear organization and functions. During mitosis, it is associated with spindle microtubule organization and chromosome positioning. Therefore, this antibody is useful as a cell cycle marker. |
| Immunogen | Full-length recombinant human NuMA expressed in insect Sf9 cells using baculovirus expression system, then purified to near homogeneity prior to immunization. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| This antibody may be used for Western blotting, immunoprecipitation, and immunofluorescence (frozen sections tested). A band at 220 kDa can be detected by Western blot. Other applications have not been tested.Suggested working dilutions: *Western Blot - 1:1,000 Immunofluorescence - 1:500 *The investigator should determine the optimal working dilution for a specific application.: |
|
| Species Reactivity | HumanMammalian species |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NUMA |
|---|---|
| Target | NuMA |
| Tissue Specificity | This antibody is specific for NuMA. |
Application
Immunocytochemistry/Immunofluorescence: NuMA Antibody [ - Immunofluorescent image of human HeLa cells fixed with 3.5% formaldehyde and stained with at 1:1000 dilution. The staining pattern shows the typical pattern for NuMA in the cell nucleus during interphase and on spindles during mitosis
Application
Western Blot: NuMA Antibody [ - Western blot analysis of NuMA expression in Cos7 whole cell lysate| Positive Control | HeLa whole cells |
|---|---|
| Application Notes | This antibody may be used for Western blotting, immunoprecipitation, and immunofluorescence (frozen sections tested). A band at 220 kDa can be detected by Western blot. Other applications have not been tested.Suggested working dilutions: *Western Blot - 1:1,000 Immunofluorescence - 1:500 *The investigator should determine the optimal working dilution for a specific application.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | 0.1 ml of whole rabbit antisera. Contains 0.1% sodium azide. |
