WB
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, All lanes: NUMA1 antibody at 3.2µg/ml. Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.IHC
Immunohistochemistry of paraffin-embedded human breast cancer using NUMA1 Antibody at dilution of 1:100.ICC/IF
Immunofluorescence staining of MCF-7 cells with NUMA1 Antibody at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-NUMA1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Nuclear mitotic apparatus protein 1 protein (627-870AA). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:5000 IHC:1:20-1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | NUMA1 |
|---|---|
| Gene Synonyms | NUMA NMP-22 |
| Gene Full Name | nuclear mitotic apparatus protein 1 |
| Gene Summary | This gene encodes a large protein that forms a structural component of the nuclear matrix. The encoded protein interacts with microtubules and plays a role in the formation and organization of the mitotic spindle during cell division. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 have been detected in patients with acute promyelocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013] |
| Alternative Names | Centrophilin stabilizes mitotic spindle in mitotic cells antibody NMP 22 antibody Nuclear matrix protein 22 antibody Nuclear mitotic apparatus protein 1 antibody Nuclear mitotic apparatus protein antibody NUMA 1 antibody NUMA antibody NuMA protein antibody NUMA1 antibody NUMA1_HUMAN antibody SP H antigen antibody SP-H antigen antibody Structural nuclear protein antibody |
| Molecular Weight(MW) | 239kDa |
| Cellular Localization | Cell membrane,Chromosome,Cytoplasm,Cytoskeleton,Membrane,Microtubule,Nucleus. |
WB
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, All lanes: NUMA1 antibody at 3.2µg/ml. Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.
IHC
Immunohistochemistry of paraffin-embedded human breast cancer using NUMA1 Antibody at dilution of 1:100.
ICC/IF
Immunofluorescence staining of MCF-7 cells with NUMA1 Antibody at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:5000 IHC:1:20-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300,Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
