Application
Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with NuMA antibody [N1], N-term diluted at 1:8000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
NuMA antibody [N1], N-term detects NuMA protein at nucleus in human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. NuMA antibody [N1], N-term diluted at 1:500.Application
NuMA antibody [N1], N-term detects NuMA protein at nucleus in human esophageal cancer by immunohistochemical analysis. Sample: Paraffin-embedded human esophageal cancer. NuMA antibody [N1], N-term diluted at 1:500.Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with NuMA antibody [N1], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using NuMA antibody at 1:100 dilution.Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using NuMA antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Product Name | NuMA antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NuMA (nuclear mitotic apparatus protein 1) |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human NuMA. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (81%), Rat (83%), Bovine (89%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NUMA antibody NUMA1 antibody nuclear mitotic apparatus protein 1 antibody centrophilin stabilizes mitotic spindle in mitotic cells antibody SP-H antigen antibody structural nuclear protein antibody |
|---|---|
| Molecular Weight(MW) | 238 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with NuMA antibody [N1], N-term diluted at 1:8000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
NuMA antibody [N1], N-term detects NuMA protein at nucleus in human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. NuMA antibody [N1], N-term diluted at 1:500.
Application
NuMA antibody [N1], N-term detects NuMA protein at nucleus in human esophageal cancer by immunohistochemical analysis. Sample: Paraffin-embedded human esophageal cancer. NuMA antibody [N1], N-term diluted at 1:500.
Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with NuMA antibody [N1], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using NuMA antibody at 1:100 dilution.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using NuMA antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Positive Control | 293T , A431 , HeLa , HepG2 , HeLa , HeLa (100 ng/ml Nocodazole treatment for 24 hr) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
