Application
Immunocytochemistry/Immunofluorescence: NUMB Antibody [ - The NUMB antibody was tested in HepG2 cells at a 1:500 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.Application
Western Blot: NUMB Antibody [ - Detection of NUMB isoforms 1 and 2 in A431 whole cell lysate (20 ug) using 0.5 ug/ml of ECL detection: 30 seconds.| Product Name | Numb antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to NumbNumb is an evolutionarily conserved membrane-associated protein. It exists in four isoforms that differ in two functional domains: a phosphotyrosine-binding (PTB) domain and a proline-rich region (PRR). It plays a role in the control of cell fate decisions during development and antagonizes the actions of Notch. Numb proteins appear to control the self-renewal of all neural progenitor cells and could possibly play similar roles in stem-cell maintenance in other tissues. |
| Immunogen | A synthetic peptide made to a C-terminal region of the murine numb protein sequence (between residues 600-653). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| By Western blot one may see any or all of the four isoforms. The molecular weight of human and mouse numb is 72 kDa (isoform 1), 66 kDa (isoform 2), 71 kDa (isoform 3), and 65 kDa (isoform 4). Multiple non-specific bands may be seen with lower dilutions and/or longer exposure times than suggested in the protocol we used to obtain our data. No other applications have been tested.Recommended Starting Dilutions:*Western Blot: 0.2-0.5 ug/mlImmunoprecipitation: Not testedImmunohisto/cytochemistry: Not tested*Optimal working dilutions should be determined by the investigator.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Synonyms | m-Nb m-numb mnb m Nb m numb mnumb |
|---|---|
| Target | Numb |
| Tissue Specificity | This antibody is specific for numb protein. |
| Cellular Localization | Membrane-associated |
Application
Immunocytochemistry/Immunofluorescence: NUMB Antibody [ - The NUMB antibody was tested in HepG2 cells at a 1:500 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.
Application
Western Blot: NUMB Antibody [ - Detection of NUMB isoforms 1 and 2 in A431 whole cell lysate (20 ug) using 0.5 ug/ml of ECL detection: 30 seconds.| Positive Control | A431 whole cell lysate |
|---|---|
| Application Notes | By Western blot one may see any or all of the four isoforms. The molecular weight of human and mouse numb is 72 kDa (isoform 1), 66 kDa (isoform 2), 71 kDa (isoform 3), and 65 kDa (isoform 4). Multiple non-specific bands may be seen with lower dilutions and/or longer exposure times than suggested in the protocol we used to obtain our data. No other applications have been tested.Recommended Starting Dilutions:*Western Blot: 0.2-0.5 ug/mlImmunoprecipitation: Not testedImmunohisto/cytochemistry: Not tested*Optimal working dilutions should be determined by the investigator.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | 0.1 ml of affinity purified antibody in Tris-glycine and 150mM NaCl containing 0.05% sodium azide. |
