Application
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE diluted at 1:1000Application
NUP107 antibody immunoprecipitates NUP107 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of NUP107 protein expressing 293T cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of NUP107 by 3 μg of NUP107 antibody 7.5% SDS-PAGE The immunoprecipitated NUP107 protein was detected by NUP107 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.| Product Name | NUP107 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NUP107 (nucleoporin 107kDa)This gene encodes a member of the nucleoporin family. The protein is localized to the nuclear rim and is an essential component of the nuclear pore complex (NPC). All molecules entering or leaving the nucleus either diffuse through or are actively transported by the NPC. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NUP107. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Chicken, Rhesus MonkeyMouse (95%), Rat (95%), Zebrafish (80%), Xenopus laevis (84%), Chicken (87%), Rhesus Monkey (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NUP84 antibody NUP107 antibody nuclear pore complex protein Nup107 antibody nucleoporin Nup107 antibody 107 kDa nucleoporin antibody nucleoporin 107kDa antibody |
|---|---|
| Molecular Weight(MW) | 106 kDa (note) (3) |
| Cellular Localization | Nucleus , nuclear pore complex , Kinetochore |
Application
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE diluted at 1:1000
Application
NUP107 antibody immunoprecipitates NUP107 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of NUP107 protein expressing 293T cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of NUP107 by 3 μg of NUP107 antibody 7.5% SDS-PAGE The immunoprecipitated NUP107 protein was detected by NUP107 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.| Positive Control | A549 , HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
