Application
NUP50 antibody [C3], C-term detects NUP50 protein by Western blot analysis. A. 50 µg mouse testis lysate/extract 10 % SDS-PAGE NUP50 antibody [C3], C-term dilution: 1:1000Application
Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE diluted at 1:1000Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using NUP50 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.Application
Immunohistochemical analysis of paraffin-embedded human normal colon, using NUP50 antibody at 1:250 dilution.| Product Name | NUP50 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NUP50 (nucleoporin 50kDa)The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NUP50. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rhesus Monkey, Chimpanzee, BovineRhesus Monkey (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC39961 antibody NPAP60 antibody NPAP60L antibody NUP50 antibody nucleoporin Nup50 antibody nuclear pore-associated protein 60L antibody nuclear pore complex protein Nup50 antibody 50 kDa nucleoporin antibody nucleoporin 50kDa antibody |
|---|---|
| Molecular Weight(MW) | 50 kDa (note) (2) |
| Cellular Localization | Nucleus , nuclear pore complex , Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side (By similarity) |
Application
NUP50 antibody [C3], C-term detects NUP50 protein by Western blot analysis. A. 50 µg mouse testis lysate/extract 10 % SDS-PAGE NUP50 antibody [C3], C-term dilution: 1:1000
Application
Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE diluted at 1:1000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using NUP50 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.
Application
Immunohistochemical analysis of paraffin-embedded human normal colon, using NUP50 antibody at 1:250 dilution.| Positive Control | H1299 , mouse testis |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
