Application
WB analysis of HeLa, Jurkat and MCF7 lysates using NUP98 antibody at a dilution of 1:1,000.Application
IP analysis of HeLa lysates using control IgG and NUP98 antibody. The precipitates were detected by the same NUP98 antibody.| Product Name | NUP98 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [3B8-D7-H10] to NUP98Signal-mediated nuclear import and export proceed through the nuclear pore complex (NPC), which is comprised of approximately 50 unique proteins collectively known as nucleoporins. The 98 kDa nucleoporin is generated through a biogenesis pathway that involves synthesis and proteolytic cleavage of a 186 kDa precursor protein. This cleavage results in the 98 kDa nucleoporin as well as a 96 kDa nucleoporin, both of which are localized to the nucleoplasmic side of the NPC. Rat studies show that the 98 kDa nucleoporin functions as one of several docking site nucleoporins of transport substrates. The human gene has been shown to fuse to several genes following chromosome translocations in acute myelogenous leukemia (AML) and T-cell acute lymphocytic leukemia (T-ALL). This gene is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described. |
| Immunogen | Purified recombinant human NUP98 protein fragments expressed in E.coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3B8-D7-H10 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NUP196 nucleoporin 98kDa NUP96 ADIR2 |
|---|---|
| Target | NUP98 |
| Molecular Weight(MW) | 98kDa (note) |
| Cellular Localization | Nucleus ＞ nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side. |
Application
WB analysis of HeLa, Jurkat and MCF7 lysates using NUP98 antibody at a dilution of 1:1,000.
Application
IP analysis of HeLa lysates using control IgG and NUP98 antibody. The precipitates were detected by the same NUP98 antibody.| Application Notes | IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50% glycerol |
