Application
Immunoprecipitation of NUP98 protein from HeLa whole cell extracts using 5 μg of NUP98 antibody [N3C1], Internal . Western blot analysis was performed using NUP98 antibody [N3C1], Internal . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Sample (30 ug of whole cell lysate) A: JurKat 7.5% SDS PAGE diluted at 1:1000| Product Name | NUP98 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to NUP98 (nucleoporin 98kDa)Signal-mediated nuclear import and export proceed through the nuclear pore complex (NPC), which is comprised of approximately 50 unique proteins collectively known as nucleoporins. The 98 kD nucleoporin is generated through a biogenesis pathway that involves synthesis and proteolytic cleavage of a 186 kD precursor protein. This cleavage results in the 98 kD nucleoporin as well as a 96 kD nucleoporin, both of which are localized to the nucleoplasmic side of the NPC. Rat studies show that the 98 kD nucleoporin functions as one of several docking site nucleoporins of transport substrates. The human gene has been shown to fuse to several genes following chromsome translocatons in acute myelogenous leukemia (AML) and T-cell acute lymphocytic leukemia (T-ALL). This gene is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human NUP98. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, RatMouse (98%), Rat (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ADIR2 antibody NUP196 antibody NUP96 antibody NUP98 antibody nuclear pore complex protein Nup98-Nup96 antibody Nup98-Nup96 antibody GLFG-repeat containing nucleoporin antibody nucleoporin 98kDa antibody |
|---|---|
| Target | NUP98 |
| Molecular Weight(MW) | 198 kDa (note) (5) |
| Cellular Localization | Nucleus , nuclear pore complex , Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side |
Application
Immunoprecipitation of NUP98 protein from HeLa whole cell extracts using 5 μg of NUP98 antibody [N3C1], Internal . Western blot analysis was performed using NUP98 antibody [N3C1], Internal . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Sample (30 ug of whole cell lysate) A: JurKat 7.5% SDS PAGE diluted at 1:1000| Positive Control | Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
