Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NUR77 antibody at 1:500 dilution.Application
Sample (50 µg of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Western Blot analysis of NR4A1 expression in transfected 293T cell line by NR4A1 polyclonal antibody. A: Non-transfected lysate. B: NR4A1 transfected lysate. 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | NUR77 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NUR77 (nuclear receptor subfamily 4, group A, member 1)This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human NUR77. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Dog, BovineRat (94%), Dog (96%), Bovine (95%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GFRP1 antibody HMR antibody MGC9485 antibody N10 antibody NAK-1 antibody NGFIB antibody NP10 antibody NUR77 antibody TR3 antibody NR4A1 antibody orphan nuclear receptor TR3 antibody nerve growth factor IB nuclear receptor variant 1 antibody ste |
|---|---|
| Molecular Weight(MW) | 64 kDa (note) (4) |
| Cellular Localization | Nucleus |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NUR77 antibody at 1:500 dilution.
Application
Sample (50 µg of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Western Blot analysis of NR4A1 expression in transfected 293T cell line by NR4A1 polyclonal antibody. A: Non-transfected lysate. B: NR4A1 transfected lysate. 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | NUR77 tansfected 293T cell , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
