Application
Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using NXF3 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using NXF3 antibody at 1:100 dilution.Application
Sample (30 ug of whole cell lysate) A: NT2D1 B: IMR32 C: U87-MG D: MCF-7 7.5% SDS PAGE diluted at 1:10000| Product Name | NXF3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NXF3 (nuclear RNA export factor 3)This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene has shortened LRR and ubiquitin-associated domains and its RDB is unable to bind RNA. It is located in the nucleoplasm but is not associated with either the nuclear envelope or the nucleolus. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NXF3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | ChimpanzeeChimpanzee (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NXF3 antibody TAPL-3 antibody TAP-like protein 3 antibody nuclear RNA export factor 3 antibody |
|---|---|
| Molecular Weight(MW) | 60 kDa (note) (2) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using NXF3 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using NXF3 antibody at 1:100 dilution.
Application
Sample (30 ug of whole cell lysate) A: NT2D1 B: IMR32 C: U87-MG D: MCF-7 7.5% SDS PAGE diluted at 1:10000| Positive Control | NT2D1 , IMR32 , U87-MG , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
