Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Occludin antibody at 1:200 dilution.Application
Sample (30 µg of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Occludin antibody detects Occludin protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: Occludin protein stained by Occludin antibody diluted at 1:250. Blue: Fluoroshield with DAPI .| Product Name | Occludin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to OccludinThis gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction. The possibility of several alternatively spliced products has been suggested but the full nature of these products has not been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Occludin. The exact sequence is proprietary. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-FIHC-PWB |
| WB:1:500~1:2000 IHC-p:1:50~1:200 IHC-f:1:100~1:500 *Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMonkeyMouseRat |
| Predicted Reactivity | Mouse, Dog, RatMouse (89%), Dog (89%), Rat (87%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BLCPMG antibody OCLN antibody occludin antibody tight junction protein occludin TM4 minus antibody |
|---|---|
| Molecular Weight(MW) | 59 kDa (note) (3) |
| Cellular Localization | Membrane; Multi-pass membrane protein , Cell junction , tight junction |
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Occludin antibody at 1:200 dilution.
Application
Sample (30 µg of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Occludin antibody detects Occludin protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: Occludin protein stained by Occludin antibody diluted at 1:250. Blue: Fluoroshield with DAPI .| Positive Control | HepG2 |
|---|---|
| Application Notes | WB:1:500~1:2000 IHC-p:1:50~1:200 IHC-f:1:100~1:500 *Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
