Application
Anti-OCT4 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with OCT4Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OCT4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OCT4.Application
Immunohistochemical staining of paraffin-embedded human lung tissue using anti-OCT4 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded human ovary tissue using anti-OCT4 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded human endometrium tissue using anti-OCT4 mouse monoclonal antibody.| Product Name | Oct4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [9B7] to OCT4Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. |
| Immunogen | Recombinant protein expressed in E.coli corresponding to amino acids 1-360 of human OCT4 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 9B7 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| ICC/IF (1:100): |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | OCT3 MGC22487 OTF4 Oct4 OTF3 POU5F1 |
|---|---|
| Target | OCT4 |
| Tissue Specificity | is specific for amino acids 1-360 of human OCT4 |
| Cellular Localization | Expressed in a diffuse and slightly punctuate pattern. |
Application
Anti-OCT4 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with OCT4
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OCT4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OCT4.
Application
Immunohistochemical staining of paraffin-embedded human lung tissue using anti-OCT4 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded human ovary tissue using anti-OCT4 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded human endometrium tissue using anti-OCT4 mouse monoclonal antibody.| Application Notes | ICC/IF (1:100): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
Data sheet for OM266262