Application
Western blot analysis of lysed extracts from human umbilical cord mesenchymal stem cell using OCT-4 Antibody.| Product Name | OCT-4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | OCT3, OCT4, OTF3, OTF4, OTF-3, Oct-3, Oct-4, CDKN6, Otc3 |
| Product description | Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency1) Pesce, M. and Scholer, H. R. (2001) Stem Cells 19, 271-278. Pan, G. and Thomson, J. A. (2007) Cell Res 17, 42-9. Cauffman, G. et al. (2006) Stem Cells 24, 2685-91. |
| Immunogen | OCT-4 antibody was raised against a peptide sequence around aa.232~236 (R-K-R-T-S) derived from OCT-4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | POU5F1 |
|---|---|
| Alternative Names | OCT3 OCT4 OTF3 OTF4 OTF-3 Oct-3 Oct-4 CDKN6 Otc3 |
| Molecular Weight(MW) | 45 kDa |
| Tissue Specificity | This antibody detects endogenous level of total OCT-4 protein. |
| Entrez Gene | 5460 |
|---|---|
| Protein Accession | NP_002692.2 |
Application
Western blot analysis of lysed extracts from human umbilical cord mesenchymal stem cell using OCT-4 Antibody.| Application Notes | Western Blot: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM286806