WB
WB analysis using OCT4/POU5F1 Monoclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with OCT4 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.WB
WB analysis of human brain using OCT4/POU5F1 Monoclonal antibody. human brain tissue were subjected to SDS PAGE followed by western blot with OCT4 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.| Product Name | OCT4/POU5F1 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human OCT4 protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:5000-1:20000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | POU5F1 |
|---|---|
| Gene Synonyms | OCT3 OCT4 OTF3 OTF4 OTF-3 Oct-3 Oct-4 Oct3/4 OCT4Borf1 |
| Gene Full Name | POU class 5 homeobox 1 |
| Gene Summary | This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013] |
| Alternative Names | OCT4 POU5F1 1C4B6 OCT3 Oct-3 |
| Molecular Weight(MW) | 39 kDa(Observed band size: 50-52 kDa) |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
WB analysis using OCT4/POU5F1 Monoclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with OCT4 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB
WB analysis of human brain using OCT4/POU5F1 Monoclonal antibody. human brain tissue were subjected to SDS PAGE followed by western blot with OCT4 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.| Application Notes | WB:1:5000-1:20000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
