WB
Western Blot. Positive WB detected in: HepG2 whole cell lysate. All lanes: OCT4 antibody at 1:500. Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution.WB
Western Blot. Positive WB detected in: Mouse brain tissue, Rat brain tissue. All lanes: OCT4 antibody at 1:1000, 1:5000, 1:8000. Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution.IHC
IHC image of Mouse anti-OCT4 Monoclonal antibody diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.ICC/IF
Immunofluorescence staining of A549 cells with Mouse anti-OCT4 Monoclonal antibody at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).FC
Overlay histogram showing A549 cells stained with Mouse anti-OCT4 Monoclonal antibody (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.| Product Name | Mouse anti-OCT4 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human POU domain, class 5, transcription factor 1 protein (1-360AA). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:10000 IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | POU5F1 |
|---|---|
| Gene Synonyms | POU class 5 homeobox 1 |
| Gene Full Name | OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4; Oct3/4; OCT4Borf1 |
| Gene Summary | This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013] |
| Alternative Names | Octamer binding transcription factor 4 antibody MGC22487 antibody Oct 3 antibody Oct 4 antibody Oct-3 antibody Oct-4 antibody OCT3 antibody Oct4 antibody Octamer binding protein 3 antibody Octamer binding protein 4 antibody Octamer binding transcription factor 3 antibody Octamer-binding protein 3 antibody Octamer-binding protein 4 antibody Octamer-binding transcription factor 3 antibody OTF 3 antibody OTF 4 antibody OTF-3 antibody OTF3 antibody OTF4 antibody PO5F1_HUMAN antibody POU class 5 homeobox 1 antibody POU domain class 5 transcription factor 1 antibody POU domain transcription factor OCT4 antibody POU domain class 5 transcription factor 1 antibody POU-type homeodomain-containing DNA-binding protein antibody POU5F1 antibody |
| Molecular Weight(MW) | 39, 31 kDa(Observed band size:45 kDa) |
| Cellular Localization | Cytoplasm. Nucleus. |
WB
Western Blot. Positive WB detected in: HepG2 whole cell lysate. All lanes: OCT4 antibody at 1:500. Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution.
WB
Western Blot. Positive WB detected in: Mouse brain tissue, Rat brain tissue. All lanes: OCT4 antibody at 1:1000, 1:5000, 1:8000. Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution.
IHC
IHC image of Mouse anti-OCT4 Monoclonal antibody diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
ICC/IF
Immunofluorescence staining of A549 cells with Mouse anti-OCT4 Monoclonal antibody at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
FC
Overlay histogram showing A549 cells stained with Mouse anti-OCT4 Monoclonal antibody (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.| Application Notes | WB:1:500-1:10000 IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
