WB
Western blot analysis was performed on O-GlcNAc modified proteins in HeLa nuclear extract (A), A549 whole cell lysate (B), and mouse brain tissue extract (C).| Product Name | O-GlcNAc antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [CTD110.6] to O-GlcNAcThis gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Oct 2009] |
| Immunogen | Synthetic peptide containing serine-O-GlcNAc |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM,k |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:100-1:1000 |
|
| Species Reactivity | All |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | O-linked N-acetylglucosamine (GlcNAc) transferase O-GLCNAC HRNT1 OGT |
|---|---|
| Target | O-GlcNAc |
WB
Western blot analysis was performed on O-GlcNAc modified proteins in HeLa nuclear extract (A), A549 whole cell lysate (B), and mouse brain tissue extract (C).| Application Notes | WB:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
