Application
Extracts of HeLa cell lysate (Lane 1), HeLa nuclear (Lane 2) and recombinant OGT expressed from baculovirus system (Lane 3) were separated on SDS-PAGE and blotted with Rabbit Anti-OGLcNAc transferase (OGT), IgG fraction and affinity-purified antibodies . The antibodies were developed with Goat Anti-Rabbit IgG, Peroxidase conjugate and chemiluminescent substrate.| Product Name | O-GlcNAc transferase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to O-GlcNAc transferaseO-linked N-acetylglucosamine transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. |
| Immunogen | Synthetic peptide: amino acids 740-755 of Human O-Linked N-Acetylglucosamine, conjugated to KLH via an N-terminal added cysteine residue. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| For IP: Use at a concentration of 2.5-5 μg/ml. For WB: Use at a concentration of 1-2 μg/ml. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Target | O-Linked N-Acetylglucosamine Transferase |
|---|
Application
Extracts of HeLa cell lysate (Lane 1), HeLa nuclear (Lane 2) and recombinant OGT expressed from baculovirus system (Lane 3) were separated on SDS-PAGE and blotted with Rabbit Anti-OGLcNAc transferase (OGT), IgG fraction and affinity-purified antibodies . The antibodies were developed with Goat Anti-Rabbit IgG, Peroxidase conjugate and chemiluminescent substrate.| Positive Control | Hela cell nuclear extracts, HEK 293-T cells |
|---|---|
| Application Notes | For IP: Use at a concentration of 2.5-5 μg/ml. For WB: Use at a concentration of 1-2 μg/ml. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4oC short term. For extended storage aliquot and store at -20oC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS, pH7.4 containing 15mM sodium azide |
