Application
O-GlcNAc transferase antibody [GT6G8] detects O-GlcNAc transferase protein by western blot analysis. A. 50 µg mouse brain lysate/extract 7.5% SDS-PAGE O-GlcNAc transferase antibody [GT6G8] dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
O-GlcNAc transferase antibody [GT6G8] detects O-GlcNAc transferase protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 7.5% SDS-PAGE O-GlcNAc transferase antibody [GT6G8] dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
O-GlcNAc transferase antibody [GT678] detects O-GlcNAc transferase protein at nucleus (mainly) and cytoplasm (less) by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: O-GlcNAc transferase protein stained by O-GlcNAc transferase antibody [GT678] diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | O-GlcNAc transferase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT678] to O-GlcNAc transferase (O-linked N-acetylglucosamine (GlcNAc) transferase)O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human O-GlcNAc transferase. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | GT678 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Pig, BovineXenopus laevis (98%), Pig (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | O-linked N-acetylglucosamine (GlcNAc) transferase O-GLCNAC OGT HRNT1 |
|---|---|
| Target | O-GlcNAc transferase |
| Cellular Localization | Cytoplasm (Potential) , Nucleus |
Application
O-GlcNAc transferase antibody [GT6G8] detects O-GlcNAc transferase protein by western blot analysis. A. 50 µg mouse brain lysate/extract 7.5% SDS-PAGE O-GlcNAc transferase antibody [GT6G8] dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
O-GlcNAc transferase antibody [GT6G8] detects O-GlcNAc transferase protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 7.5% SDS-PAGE O-GlcNAc transferase antibody [GT6G8] dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
O-GlcNAc transferase antibody [GT678] detects O-GlcNAc transferase protein at nucleus (mainly) and cytoplasm (less) by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: O-GlcNAc transferase protein stained by O-GlcNAc transferase antibody [GT678] diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | PC-12 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
