Application
Olfactomedin-like 3 antibody [N1C1] detects Olfactomedin-like 3 protein at cytoplasm in mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Olfactomedin-like 3 antibody [N1C1] diluted at 1:500.Application
Immunofluorescence analysis of methanol-fixed A549, using Olfactomedin-like 3 antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | OLFML3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to OLFML3 (olfactomedin-like 3)Secreted scaffold protein that plays an essential role in dorsoventral patterning during early development. Stabilizes axial formation by restricting chordin (CHRD) activity on the dorsal side. Acts by facilitating the association between the tolloid proteases and their substrate chordin (CHRD), leading to enhance chordin (CHRD) degradation (By similarity). May have matrix-related function involved in placental and embryonic development, or play a similar role in other physiological processes. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human OLFML3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Rhesus Monkey, BovineRat (93%), Rhesus Monkey (99%), Bovine (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HNOEL-iso antibody OLF44 antibody OLFML3 antibody olfactomedin-like protein 3 antibody hOLF44 antibody olfactomedin-like 3 antibody |
|---|---|
| Molecular Weight(MW) | 46 kDa (note) (2) |
| Cellular Localization | Secreted |
Application
Olfactomedin-like 3 antibody [N1C1] detects Olfactomedin-like 3 protein at cytoplasm in mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Olfactomedin-like 3 antibody [N1C1] diluted at 1:500.
Application
Immunofluorescence analysis of methanol-fixed A549, using Olfactomedin-like 3 antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A549 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
