Application
Optineurin antibody immunoprecipitates Optineurin protein in IP experiments. IP samples: HepG2 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of Optineurin protein by 4 μg Optineurin antibody 7.5 % SDS-PAGE The immunoprecipitated Optineurin protein was detected by Optineurin antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Optineurin antibody at 1:500 dilution.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Optineurin antibody at 1:200 dilution.Application
Sample(30 µg whole cell lysate) A:A431 B:H1299 C:Hep G2 10% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Optineurin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Optineurin (optineurin)This gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Optineurin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Rhesus Monkey, BovineMouse (82%), Rat (83%), Pig (85%), Rhesus Monkey (96%), Bovine (86%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ALS12 antibody FIP2 antibody GLC1E antibody HIP7 antibody HYPL antibody NRP antibody TFIIIA-INTP antibody OPTN antibody HIP-7 antibody optic neuropathy-inducing protein antibody tumor necrosis factor alpha-inducible cellular protein containing le |
|---|---|
| Molecular Weight(MW) | 66 kDa (note) (4) |
| Cellular Localization | Cytoplasm , perinuclear region , Golgi apparatus |
Application
Optineurin antibody immunoprecipitates Optineurin protein in IP experiments. IP samples: HepG2 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of Optineurin protein by 4 μg Optineurin antibody 7.5 % SDS-PAGE The immunoprecipitated Optineurin protein was detected by Optineurin antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Optineurin antibody at 1:500 dilution.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Optineurin antibody at 1:200 dilution.
Application
Sample(30 µg whole cell lysate) A:A431 B:H1299 C:Hep G2 10% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
