Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of methanol-fixed HeLa, using ORP1 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ORP1 antibody at 1:100 dilution.| Product Name | ORP1 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ORP1 (oxysterol binding protein-like 1A)This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although some members contain only the sterol-binding domain. Transcript variants derived from alternative promoter usage and/or alternative splicing exist; they encode different isoforms. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ORP1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | RatRat (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ10217 antibody ORP-1 antibody ORP1 antibody OSBPL1B antibody OSBPL1A antibody OSBP-related protein 1 antibody oxysterol-binding protein-related protein 1 variant 1 antibody oxysterol-binding protein-related protein 1 variant 2 antibody oxysterol |
|---|---|
| Molecular Weight(MW) | 108 kDa (note) (3) |
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of methanol-fixed HeLa, using ORP1 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ORP1 antibody at 1:100 dilution.| Positive Control | A431 , H1299 , HeLa , HepG2 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
