Application
Immunofluorescence analysis of methanol-fixed Hep3B, using ORP150 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using ORP150 antibody at 1:100 dilution.Application
ORP150 antibody [C2C3], C-term detects ORP150 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ORP150 protein stained by ORP150 antibody [C2C3], C-term diluted at 1:200. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.Application
ORP150 antibody [C2C3], C-term detects ORP150 protein at cytoplasm by immunofluorescent analysis. Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ORP150 protein stained by ORP150 antibody [C2C3], C-term diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.Application
ORP150 antibody detects ORP150 protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 5% SDS-PAGE, and blotted with ORP150 antibody diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
ORP150 antibody detects ORP150 protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 5% SDS-PAGE, and blotted with ORP150 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
ORP150 antibody detects ORP150 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and blotted with ORP150 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
ORP150 antibody [C2C3], C-term detects ORP150 protein at cytoplasm on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. ORP150 antibody [C2C3], C-term diluted at 1:500.| Product Name | ORP150 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ORP150 (hypoxia up-regulated 1)The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human ORP150. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | BovineBovine (88%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686N08236 antibody FLJ94899 antibody FLJ97572 antibody Grp170 antibody HSP12A antibody ORP150 antibody HYOU1 antibody hypoxia up-regulated protein 1 antibody glucose-regulated protein 170 antibody ORP-150 antibody 150 kDa oxygen-regulated pr |
|---|---|
| Molecular Weight(MW) | 111 kDa (note) (2) |
| Cellular Localization | Endoplasmic reticulum lumen |
Application
Immunofluorescence analysis of methanol-fixed Hep3B, using ORP150 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using ORP150 antibody at 1:100 dilution.
Application
ORP150 antibody [C2C3], C-term detects ORP150 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ORP150 protein stained by ORP150 antibody [C2C3], C-term diluted at 1:200. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.
Application
ORP150 antibody [C2C3], C-term detects ORP150 protein at cytoplasm by immunofluorescent analysis. Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ORP150 protein stained by ORP150 antibody [C2C3], C-term diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.
Application
ORP150 antibody detects ORP150 protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 5% SDS-PAGE, and blotted with ORP150 antibody diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
ORP150 antibody detects ORP150 protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 5% SDS-PAGE, and blotted with ORP150 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
ORP150 antibody detects ORP150 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and blotted with ORP150 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
ORP150 antibody [C2C3], C-term detects ORP150 protein at cytoplasm on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. ORP150 antibody [C2C3], C-term diluted at 1:500.| Positive Control | MCF-7 , MDA-MB-231 , mouse liver , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
