IHC
Immunohistochemical staining of osteogenic markers (Runx2, OCN and COL-1).IHC
The immumohistochemical staining for detecting the expression of Runx2, OCN and COL-1 at 4 (A) and 12 (B) weeks (400 ×).| Product Name | Osteocalcin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [OC4 - 30 ] to OsteocalcinOsteocalcin belongs to the osteocalcin / matrix Gla-protein family and constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. |
| Immunogen | Full length protein (Cow). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | OC4-30 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHCIHC-FIHC-PWB |
| ELISA: Use at an assay dependent dilution. IHC-P: Use at a concentration of 10 μg/ml. IHC-Fr: Use at a concentration of 10 μg/ml. WB: Use at an assay dependent dilution. Predicted molecular weight: 12.0 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | BovineDogGoatHumanPigRabbitRatSheep |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | BGP |
|---|---|
| Target | Osteocalcin |
| Cellular Localization | Secreted |
IHC
Immunohistochemical staining of osteogenic markers (Runx2, OCN and COL-1).
IHC
The immumohistochemical staining for detecting the expression of Runx2, OCN and COL-1 at 4 (A) and 12 (B) weeks (400 ×).| Application Notes | ELISA: Use at an assay dependent dilution. IHC-P: Use at a concentration of 10 μg/ml. IHC-Fr: Use at a concentration of 10 μg/ml. WB: Use at an assay dependent dilution. Predicted molecular weight: 12.0 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: 1% BSA, 10mM PBS. pH 7.4 |
