Application
staining Osteopontin in human breast carcinoma by Immunohistochemistry (FFPE-sections).Application
Human Breast Carcinoma stained with anti-Osteopontin antibody| Product Name | Osteopontin antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Osteopontin predilutedOsteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. OPN is multifunctional, with activities in cell migration, cell survival, inhibition of calcification, regulation of immune cell function, and control of tumor cell phenotype. Targeting of the gene encoding Osteopontin, has revealed that while it is not necessary for normal embryonic development, fertility, and health under pathogen-free conditions, loss of the protein has significant consequences in several models of injury/disease as diverse as renal injury, viral and bacterial infection, bone remodeling, and tumor growth. The fact that no other proteins seem to share a redundant activity with Osteopontin under these conditions suggests that it has a unique functional role during tissue injury and stress. |
| Immunogen | A synthetic peptide derived from internal domain of human Osteopontin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| IHC-P: Use at an assay dependent dilution. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanDogPig |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BNSP BSPI ETA-1 MGC110940 OPN ETA 1 ETA1 |
|---|---|
| Target | Osteopontin |
| Cellular Localization | Secreted |
Application
staining Osteopontin in human breast carcinoma by Immunohistochemistry (FFPE-sections).
Application
Human Breast Carcinoma stained with anti-Osteopontin antibody| Positive Control | Breast carcinoma |
|---|---|
| Application Notes | IHC-P: Use at an assay dependent dilution. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution and store at 4°C. Do not freeze. |
| Storage Buffer | PBS, pH 7.4 containing 1%BSA and 0.1% sodium azide |
