Application
Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with OTUB1 antibody diluted at 1:1000.| Product Name | OTUB1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to OTUB1 (OTU domain, ubiquitin aldehyde binding 1)Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of removing NEDD8 from NEDD8 conjugates, but with a nuch lower preference compared to 'Lys-48'-linked ubiquitin. |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of mouse OTUB1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | MouseRat |
| Predicted Reactivity | Human, Xenopus laevis, Dog, Pig, BovineHuman (98%), Xenopus laevis (88%), Dog (98%), Pig (98%), Bovine (100%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AI850305 antibody OTU domain ubiquitin aldehyde binding 1 antibody |
|---|---|
| Target | OTUB1 |
| Molecular Weight(MW) | 31 kDa (note) |
Application
Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with OTUB1 antibody diluted at 1:1000.| Positive Control | mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
