Application
Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with OTUB1 antibody [N1C1] diluted at 1:500.Application
Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 12% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of methanol-fixed HeLa, using OTUB1 antibody at 1:50 dilution.| Product Name | OTUB1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to OTUB1 (OTU domain, ubiquitin aldehyde binding 1)The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human OTUB1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Pig, BovineDog (99%), Pig (100%), Bovine (100%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ20113 antibody FLJ40710 antibody MGC111158 antibody MGC4584 antibody OTB1 antibody OTU1 antibody OTUB1 antibody otubain-1 antibody ubiquitin thioesterase OTUB1 antibody deubiquitinating enzyme OTUB1 antibody ubiquitin-specific protease otubain |
|---|---|
| Molecular Weight(MW) | 31 kDa (note) |
Application
Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with OTUB1 antibody [N1C1] diluted at 1:500.
Application
Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 12% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of methanol-fixed HeLa, using OTUB1 antibody at 1:50 dilution.| Positive Control | HeLa , HepG2 , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
