Application
HEK293T cells transfected with either RC209650 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-OTUB2 antibody, and then analyzed by flow cytometry.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OTUB2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OTUB2.Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-OTUB2 monoclonal antibody.| Product Name | OTUB2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [11A5] to OTUB2Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains. |
| Immunogen | Full length human recombinant protein of human OTUB2 (NP_075601) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 11A5 |
| Host Species | Mouse |
| Tested Applications | FACSWB |
| Flow Cytometry 1:100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC3102 C14orf137 OTB2 OTUB2 FLJ21916 OTU2 |
|---|---|
| Target | OTUB2 |
| Tissue Specificity | is specific for human OTUB2 (NP_075601). |
Application
HEK293T cells transfected with either RC209650 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-OTUB2 antibody, and then analyzed by flow cytometry.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OTUB2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OTUB2.
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-OTUB2 monoclonal antibody.| Application Notes | Flow Cytometry 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
