Application
Sample (10 µg of whole cell lysate) A: Hep3B 6% SDS PAGE diluted at 1:2500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
P Glycoprotein antibody detects P Glycoprotein protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: P Glycoprotein protein stained by P Glycoprotein antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | P Glycoprotein antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to P Glycoprotein (ATP-binding cassette, sub-family B (MDR/TAP), member 1)The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human P Glycoprotein. The exact sequence is proprietary. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Cat, Rabbit, Sheep, Rhesus MonkeyCat (92%), Rabbit (93%), Sheep (93%), Rhesus Monkey (93%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ABC20 antibody CD243 antibody CLCS antibody GP170 antibody MDR1 antibody MGC163296 antibody P-GP antibody PGY1 antibody ABCB1 antibody doxorubicin resistance antibody P-glycoprotein 1 antibody multidrug resistance protein 1 antibody colchicin s |
|---|---|
| Molecular Weight(MW) | 141 kDa (note) |
Application
Sample (10 µg of whole cell lysate) A: Hep3B 6% SDS PAGE diluted at 1:2500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
P Glycoprotein antibody detects P Glycoprotein protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: P Glycoprotein protein stained by P Glycoprotein antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | Hep3B |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
