Application
BCAR1 antibody [N2C2], Internal detects BCAR1 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. BCAR1 antibody [N2C2], Internal diluted at 1:500.Application
BCAR1 antibody [N2C2], Internal detects BCAR1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: BCAR1 protein stained by BCAR1 antibody [N2C2], Internal diluted at 1:500. Blue: Hoechst 33342 staining.Application
Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000| Product Name | p130Cas antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to p130Cas (breast cancer anti-estrogen resistance 1)BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BCAR1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (88%), Bovine (88%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CAS antibody CAS1 antibody CASS1 antibody CRKAS antibody FLJ12176 antibody FLJ45059 antibody P130Cas antibody BCAR1 antibody Crk-associated substrate p130Cas antibody breast cancer anti-estrogen resistance protein 1 antibody Cas scaffolding prote |
|---|---|
| Molecular Weight(MW) | 93 kDa (note) (8) |
| Cellular Localization | Cell junction , focal adhesion , Cytoplasm |
Application
BCAR1 antibody [N2C2], Internal detects BCAR1 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. BCAR1 antibody [N2C2], Internal diluted at 1:500.
Application
BCAR1 antibody [N2C2], Internal detects BCAR1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: BCAR1 protein stained by BCAR1 antibody [N2C2], Internal diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000| Positive Control | H1299 , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
