Application
Western blot analysis using CDKN2A mAb against HEK293 (1) and CDKN2A (AA: 1-156)-hIgGFc transfected HEK293 (2) cell lysate.Application
Immunohistochemical analysis of paraffin-embedded lung cancer tissues using CDKN2A mouse mAb with DAB staining.Application
Flow cytometric analysis of HEK293 cells using CDKN2A mouse mAb (green) and negative control (red).Application
WB analysis of human p14ARF recombinant protein using p14ARF antibody [1D7D2].Application
IHC-P analysis of endometrial cancer tissue using p14ARF antibody [1D7D2].Application
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);| Product Name | p14ARF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1D7D2] to p14ARFThis gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 cont |
| Immunogen | The immunogen corresponding to a region within amino acids 1 and 156 of human CDKN2A expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1D7D2 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TP16 P14ARF ARF P16-INK4A INK4A P16INK4A P14 MLM CDKN2 CDK4I MTS-1 P16 CMM2 INK4 P16INK4 P |
|---|---|
| Target | p14ARF |
| Molecular Weight(MW) | 16.5 kDa (note) |
Application
Western blot analysis using CDKN2A mAb against HEK293 (1) and CDKN2A (AA: 1-156)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Immunohistochemical analysis of paraffin-embedded lung cancer tissues using CDKN2A mouse mAb with DAB staining.
Application
Flow cytometric analysis of HEK293 cells using CDKN2A mouse mAb (green) and negative control (red).
Application
WB analysis of human p14ARF recombinant protein using p14ARF antibody [1D7D2].
Application
IHC-P analysis of endometrial cancer tissue using p14ARF antibody [1D7D2].
Application
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
