Application
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with p18 INK4c antibody diluted at 1:500.Application
Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membranes were blotted with p18 INK4c antibody diluted at 1:500 and competitor's antibody (SC-865) diluted by 1:100. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | p18 INK4c antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to p18 INK4c (cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4))The protein encoded by this gene is a member of the INK4 family of cyclin-dependent kinase inhibitors. This protein has been shown to interact with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. Ectopic expression of this gene was shown to suppress the growth of human cells in a manner that appears to correlate with the presence of a wild-type RB1 function. Studies in the knockout mice suggested the roles of this gene in regulating spermatogenesis, as well as in suppressing tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode an identical protein, have been reported. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human p18 INK4c. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Rhesus Monkey, BovineHuman (100%), Rat (88%), Rhesus Monkey (100%), Bovine (89%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | p18 p18-INK4C cyclin-dependent kinase inhibitor 2C (p18 inhibits CDK4) CDKN2C INK4C |
|---|---|
| Target | p18 INK4c |
| Molecular Weight(MW) | 18 kDa (note) (2) |
Application
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with p18 INK4c antibody diluted at 1:500.
Application
Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membranes were blotted with p18 INK4c antibody diluted at 1:500 and competitor's antibody (SC-865) diluted by 1:100. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
