Application
Primary antibody (at 1:200) used to detect p21 expression by western blot. Samples 15µg each of A) A431 lysate B) HL-60 lysate C) Hela lysate| Product Name | p21 Cip1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to p21 Cip1Cell cycle progression is regulated by cyclins and their cognate Cdks (1-3). p21 (WAF1/Cip1) inhibits the activity of each member of the cyclin/Cdk family and over expression of this protein inhibits the proliferation of mammalian cells (5). The expression of p21 is inducible by a wide range of stress stimuli and its transcription can be enhanced by p53 (6). Another member of the CDIs is p27 (also known as Kip1), which also sees up regulation in response to antimitogenic stimuli (7). The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes, like cyclin D1/Cdk4 (4,8). An additional CDI has been found to bind Cdk4 and Cdk6, p16 (INK4A), and when such complexes are formed, the progression of the cell cycle is halted (9). It has become increasingly clear that p16 is a very important tumor suppressor gene whose frequent loss occurs early in many human cancers. p16 is a major target in carcinogenesis that is rivaled in frequency only by p53 (10). |
| Immunogen | Synthetic peptide (Human) (C terminal). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-FIPWB |
| IHC-Fr: Use at an assay dependent concentration. IP: Use at an assay dependent concentration. WB: Use at an assay dependent concentration. Can be blocked with GTX28015 p21 (C-term) peptide (human) . Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Gene Synonyms | CAP20 CDKN1 CIP1 MDA-6 P21 SDI1 WAF1 p21CIP1 MDA 6 MDA6 |
|---|---|
| Target | p21 Cip1 |
| Tissue Specificity | Reacts with p21(also designated WAF1/Cip1) |
| Cellular Localization | Nuclear |
Application
Primary antibody (at 1:200) used to detect p21 expression by western blot. Samples 15µg each of A) A431 lysate B) HL-60 lysate C) Hela lysate| Positive Control | C32 |
|---|---|
| Application Notes | IHC-Fr: Use at an assay dependent concentration. IP: Use at an assay dependent concentration. WB: Use at an assay dependent concentration. Can be blocked with GTX28015 p21 (C-term) peptide (human) . Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store this product at 4 °C, do not freeze. The product is stable for one year from the date of shipment. |
| Storage Buffer | PBS with 0.1% sodium azide |
