Application
Anti-P2X2_Receptor_(extracellular)_ - Western blot analysis of rat brain membrane (lanes 1 and 4), mouse brain membrane (lanes 2 and 5) and PC12 (lanes 3 and 6): 1-3. Anti-P2X2 Receptor (extracellular) antibody, (1:200). 4-6. Anti-P2X2 Receptor (extracellular) antibody, preincubated with the control peptide antigen.Application
Anti-P2X2_Receptor_(extracellular)_ - Expression of P2X2 in rat PC12 cells Immunocytochemical staining of intact living rat pheochromocytoma cell line (PC12). A. Extracellular staining of cells with Anti-P2X2 Receptor (extracellular) antibody, (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Staining of cells with DAPI used as the counterstain. C. Merged images of A and B.| Product Name | P2X2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to P2X2subunits assemble into ATP-gated ion channels involved in neuritogenesis and cell survival [RGD, Feb 2006] |
| Immunogen | Peptide (C)KSDYLKHSTFDQDS, corresponding to amino acid residues 207-220 of rat P2X2 with replacement of cysteine 214 (C214) with serine (*S) (Accession P49653 ). Extracellular. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P2rx2 purinergic receptor P2X ligand-gated ion channel 2 |
|---|---|
| Target | P2X2 |
Application
Anti-P2X2_Receptor_(extracellular)_ - Western blot analysis of rat brain membrane (lanes 1 and 4), mouse brain membrane (lanes 2 and 5) and PC12 (lanes 3 and 6): 1-3. Anti-P2X2 Receptor (extracellular) antibody, (1:200). 4-6. Anti-P2X2 Receptor (extracellular) antibody, preincubated with the control peptide antigen.
Application
Anti-P2X2_Receptor_(extracellular)_ - Expression of P2X2 in rat PC12 cells Immunocytochemical staining of intact living rat pheochromocytoma cell line (PC12). A. Extracellular staining of cells with Anti-P2X2 Receptor (extracellular) antibody, (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Staining of cells with DAPI used as the counterstain. C. Merged images of A and B.| Application Notes | ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3. |
