Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using P2X7 antibody at 1:250 dilution.Application
P2X7 antibody detects P2X7 protein by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: P2X7 protein stained by P2X7 antibody diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI .Application
P2X7 antibody detects P2X7 protein at cell membrane and cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. P2X7 antibody diluted at 1:500.| Product Name | P2X7 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to P2X7 (purinergic receptor P2X, ligand-gated ion channel, 7)The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants which would encode different isoforms have been identified although some fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human P2X7. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-F,IHC-P,WB |
| *Optimal dilutions/concentrations should be determined by the researcherWB:1:500~1:3000 IHC:1:50~1:500 |
|
| Species Reactivity | Human,Mouse,Rat |
| Predicted Reactivity | Dog, Bovine, Guinea pigDog (92%), Bovine (86%), Guinea pig (83%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC20089 antibody, P2X7 antibody, P2RX7 antibody, P2Z receptor antibody, P2X7 receptor antibody, P2X purinoceptor 7 antibody, ATP receptor antibody, purinergic receptor P2X7 variant A antibody, |
|---|---|
| Molecular Weight(MW) | 69 kDa (note) |
| Cellular Localization | Membrane; Multi-pass membrane protein |
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using P2X7 antibody at 1:250 dilution.
Application
P2X7 antibody detects P2X7 protein by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: P2X7 protein stained by P2X7 antibody diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI .
Application
P2X7 antibody detects P2X7 protein at cell membrane and cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. P2X7 antibody diluted at 1:500.| Positive Control | A431 , Rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcherWB:1:500~1:3000 IHC:1:50~1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
