Application
Anti-P2Y12_Receptor_(extracellular) - Indirect flow cytometry analysis of MEG-O1 living cells: ___ Unstained cells.___ Cells + Anti-P2Y12 Receptor (extracellular) antibody, (5-10 μg/1x106 cells).Application
Anti-P2Y12_Receptor_(extracellular) - Western blot analysis of rat brain membrane (lanes 1 and 3) and MEG-01 (lanes 2 and 4) cell line lysate: 1-2. Anti-P2Y12 Receptor (extracellular) antibody, (1:200). 3-4. Anti-P2Y12 Receptor (extracellular) antibody, preincubated with the control peptide antigen.Application
Anti-P2Y12_Receptor_(extracellular) - Expression of P2Y12 in rat RBL cells Immunocytochemical staining of intact living rat basophilic leukemia (RBL) cells. A. Extracellular staining of cells using Anti-P2Y12 (extracellular) antibody, (1:100) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells.| Product Name | P2Y12 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to P2Y12The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is involved in platelets aggregation, and is a potential target for the treatment of thromboembolisms and other clotting disorders. Two transcript variants encoding the same isoform have been identified for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | Peptide CTAENTLFYVKES, corresponding to amino acid residues 270-282 of human P2Y12 receptor (Accession Q9H244). 3rd extracellular loop. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFWB |
| FACS:1:200~1:500 ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BDPLT8 P2RY12 P2Y(12)R ADPG-R SP1999 purinergic receptor P2Y G-protein coupled 12 P2Y(AC) P2Y(cyc) P2T(AC) P2Y(ADP) HORK3 |
|---|---|
| Target | P2Y12 |
Application
Anti-P2Y12_Receptor_(extracellular) - Indirect flow cytometry analysis of MEG-O1 living cells: ___ Unstained cells.___ Cells + Anti-P2Y12 Receptor (extracellular) antibody, (5-10 μg/1x106 cells).
Application
Anti-P2Y12_Receptor_(extracellular) - Western blot analysis of rat brain membrane (lanes 1 and 3) and MEG-01 (lanes 2 and 4) cell line lysate: 1-2. Anti-P2Y12 Receptor (extracellular) antibody, (1:200). 3-4. Anti-P2Y12 Receptor (extracellular) antibody, preincubated with the control peptide antigen.
Application
Anti-P2Y12_Receptor_(extracellular) - Expression of P2Y12 in rat RBL cells Immunocytochemical staining of intact living rat basophilic leukemia (RBL) cells. A. Extracellular staining of cells using Anti-P2Y12 (extracellular) antibody, (1:100) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells.| Application Notes | FACS:1:200~1:500 ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3. |
