Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 10% SDS PAGE diluted at 1:1000Application
Immunoprecipitation of P2Y2 protein from 293T whole cell extracts using 5 μg of P2Y2 antibody [N3C3] . Western blot analysis was performed using P2Y2 antibody [N3C3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [N3C3] diluted at 1:1000.| Product Name | P2Y2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to P2Y2 (purinergic receptor P2Y, G-protein coupled, 2)The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is responsive to both adenosine and uridine nucleotides. It may participate in control of the cell cycle of endometrial carcinoma cells. Three transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human P2Y2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, BovinePig (82%), Bovine (82%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HP2U antibody MGC20088 antibody MGC40010 antibody P2RU1 antibody P2U antibody P2U1 antibody P2UR antibody P2Y2 antibody P2Y2R antibody P2RY2 antibody P2U nucleotide receptor antibody P2Y purinoceptor 2 antibody purinergic receptor P2Y2 antibody |
|---|---|
| Molecular Weight(MW) | 42 kDa (note) (4) |
| Cellular Localization | Cell membrane; Multi-pass membrane protein |
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 10% SDS PAGE diluted at 1:1000
Application
Immunoprecipitation of P2Y2 protein from 293T whole cell extracts using 5 μg of P2Y2 antibody [N3C3] . Western blot analysis was performed using P2Y2 antibody [N3C3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [N3C3] diluted at 1:1000.| Positive Control | 293T , A431 , Jurkat , Raji , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
